Peptide sequence around phosphorylation site of serine 303 (P-P-S(p)-P-P) derived from Human HSF1.
ELISA , WB , IHC
WB:1:500-1:1000 , IHC:1:50-1:100
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.