Peptide sequence around phosphorylation site of threonine 435(E-G-T(p)-L-S) derived from Human NFkB-p65.
Human , Mouse , Rat
ELISA , WB , IHC , IF
WB:1:500-1:1000 , IHC:1:50-1:100 , IF:1:100-1:200
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.